1-MCP处理对“东红”猕猴桃货架期品质的影响

以"东红"猕猴桃为试材,通过采后用不同浓度(0、0.25、0.50、0.75μL·L^-1)1-甲基环丙烯(1-methylcyclopropene,1-MCP)进行处理后,将果实至于(20.0±0.5)℃层析冷柜中对其进行货架期贮藏,研究"东红"猕猴桃果实品质的变化,以期为延长猕猴桃货架期品质提供参考依据。结果表明:0.75μL·L^-11-MCP能够有效地抑制"东红"猕猴桃果实硬度、b*值、超氧化物歧化酶(SOD)活性及过氧化氢酶(CAT)活性的下降;0.75μL·L^-1及0.50μL·L^-11-MCP均能够显著降低"东红"猕猴桃果实呼吸强度、乙烯生成速率和脂氧合酶(LOX)活性;0.25、0.50、0.75μL·L^-11-MCP能够更好地推迟"东红"猕猴桃果实可溶性固形物含量的上升,而不同浓度1-MCP处理间对"东红"猕猴桃果实可滴定酸含量的作用效果无显著差异。综合比较,0.75μL·L^-11-MCP能够更好地保持"东红"猕猴桃的货架期品质,其次为0.50μL·L^-11-MCP。综合考虑"东红"猕猴桃货架期品质变化及成本,建议"东红"猕猴桃的1-MCP使用浓度为0.50~0.75μL·L^-1。     

Effect of soil acidification on the growth and nitrogen use efficiency of maize in Ultisols

Purpose

To examine the effect of soil acidification on growth and nitrogen (N) uptake by maize in Ultisols.

Materials and methods

A clay Ultisol derived from Quaternary red earth and a sandy Ultisol derived from tertiary red sandstone were used in this study. A pot experiment was conducted with maize growing in the two Ultisols acidified to different pH values. Urea with ¹⁵N abundance of 10.11% was used to investigate the distribution of N fertilizer between soil and plant. Total N content and ¹⁵N abundance in plant and soil samples were determined by elemental analysis-isotope mass spectrometry.

Results and discussion

Critical soil pHs of 4.8 and 5.0 were observed for maize growing in the clay and sandy Ultisols, respectively. Below the critical soil pH, increasing soil pH significantly increased maize height and the yield of maize shoots and roots (both P < 0.05), but changes in soil pH showed no significant effect on maize growth above the critical soil pH in both Ultisols. Maize growing in the sandy Ultisol was more sensitive to changes in soil pH than in the clay Ultisol. Increase in the pH in both Ultisols also increased N accumulation in maize, the N derived from fertilizer in maize, physiological N use efficiency, and N use efficiency (NUE) by maize. Changes in soil pH had a greater effect on these parameters below the critical soil pH, compared to above. The change in soil pH had a greater effect on N accumulation in maize, the N derived from fertilizer in maize, and NUE in the sandy Ultisol than in the clay Ultisol. The NUE increased by 24.4% at pH 6.0, compared with pH 4.0 in the clay Ultisol, while the NUE at pH 5.0 was 4.8 times that at pH 4.0 in the sandy Ultisol. The increase in soil pH increased the ratio of N accumulation in maize/soil residue N and decreased the potential loss of fertilizer N from both Ultisols.

Conclusions

Soil acidification inhibited maize growth, reduced N uptake by maize, and thus, decreased NUE. To maintain soil pH of acidic soils above the critical values for crops is of practical importance for sustainable food production in acidic soils.

     

堆垛车核心零件的参数化设计与有限元分析系统

为了提高堆垛车的设计效率,减少企业对设计研发新产品的投入及推进我国堆垛车的设计创新。以堆垛车核心零件为例,利用NX11.0为平台,VS2012为开发工具,结合NX二次开发技术开发了集参数化建模以及参数化有限元分析系统,实现了堆垛车零件的自动创建及自动分析。该系统大大提高了产品的设计效率和质量,为新产品的研发做好充分准备。     

Essential amino acids supplementation to practical diets affects growth,feed utilization and glucose metabolism‐related signalling molecules of blunt snout bream (Megalobrama amblycephala)

Six diets were formulated to investigate the success of fish meal (FM) replacement by plant proteins; diet 1 reflected a commercial feed (8% FM), diet 3 contained 4% FM, and diet 5 was devoid of FM. Whereas, diet 2, diet 4, and diet 6 reflected diet 1, diet 3, diet 5, respectively, and supplemented with essential amino acid (EAA). At the end of 8‐week trial, there was no significant difference in survival rate. Significantly higher final weight, weight gain rate, and specific growth rate were recorded in the group fed diet 2 compared with the other treatments (except diet 4) (p < 0.05). Feed conversion ratio of fish fed diet 2 was significantly lower than those fed diets 1, 3, and 5 (p < 0.05). The lowest feed intake and highest protein efficiency rate were found in fish fed diet 2 (p < 0.05). There was no significant difference in whole body compositions between treatments. Plasma aspartate transaminases, alanine aminotransferase, and glucose were significantly affected by dietary treatments (p < 0.05), while plasma protein and albumin contents were not influenced by the treatments. The relative expression of target of rapamycin (TOR) and phosphatidylinositol 3‐kinase, regulatory subunit 1 (alpha) (PIK3R1) in fish fed diet 3 (4% FM) were significantly down‐regulated compared with those fed diet 6 for TOR and diets 4 and 6 for PIK3R1 (p < 0.05). Insulin receptor substrate 1 (IRS‐1) and janus kinase 3 (JAK3) expressions were fluctuated, with the higher levels in fish fed diets 4 and 6. In conclusion, the findings of this study indicate that plant protein mixture supplemented with EAA could be used to substitute FM in practical diet for Megalobrama amblycephala.     

Expression and functional characterization of glutamine synthetase from giant freshwater prawn (Macrobrachium rosenbergii) under osmotic stress

The freshwater prawn, Macrobrachium rosenbergii naturally lives in the freshwater, though it migrates to the brackish water environment during spawning that claimed to be resistant on a broad range of saline fluxes. However, little is known about the osmoregulatory patterns and the effect of an enzyme glutamine synthetase (GS) in M. rosenbergii under stress. Here, we described the identification and functional characterization of GS from M. rosenbergii (Mr‐GS) at molecular and protein levels. The identified Mr‐GS was comprised of 361 amino acids that phylogenetically shared the highest identity with other crustaceans and predicted to contain Gln‐synt_C and Gln‐synt_N domains at the respective terminal regions. Tissue distribution analysis in M. rosenbergii revealed that the Mr‐GS was highly expressed in muscle, and commonly existed in other examined tissues in the following order gills > heart > stomach > brain > haemolymph. Whereas, the mRNA of Mr‐GS was significantly up‐regulated in the muscle and gill tissues following challenges with either hyper (0 → 13‰), or hypo (13 → 0‰) osmotic stress at 3, 6 and 12 hr. Furthermore, the level of Glutamine concentration was positively correlated with the GS mRNA and protein expression patterns in hyper‐osmotic stress, whereas in hypo‐osmotic stress a slight decrease in the gills and maintained a level in the muscle tissues at 3, 6 and 12 hr post‐treatments. Our findings suggest that Mr‐GS potentially exhibited the osmoregulation responses in the gill and muscle tissues of M. rosenbergii throughout the time of osmotic stress, which will benefit for future study on osmoregulation.     

Nutrient cycling and biomass flows in a low-quality forest stand improvement system in the Lesser Khingan Range of China

The material flow and bulk internal flow analyses were used to establish a material accumulation and cycling model for a low-quality forest stand improvement system and a series of processes were considered. The model was applied in a one-hectare low-quality forest plot in the Lesser Khingan Range of China. Results showed that during 1997–2007, the stands absorbed 270.19 kg of N, 74.28 kg of P, and 124.39 kg of K from soils, 51.82 kg of N and 2.38 kg of P were directly absorbed by foliage, and 16.25 kg of K was released to soils by eluviation. Until 2007, the accumulated nutrients in the stands included 236.91 kg of N, 65.28 kg of P, and 108.55 kg of K. When horizontal strip clearcutting was applied in 2007, 50% accumulated nutrients in the stands were shifted due to harvesting operations, and 212.74 kg of N, 26.97 kg of P, and 98.88 kg of K were accumulated in soils, declining by 9.47% for N, 3.68% for P, and 17.60% for K, respectively, compared with year 1997. 94.61 t per hectare of biomass was generated, of which the biomass in stands accounted for 87.36%. The felled tree biomass was 36.89 t per hectare, of which 84.90% and 10.03% of biomass were utilized in terms of logs and other means, and the rest was left on site.     

FAHP-GIS在FAST台址单体滑坡危险性评价中的应用

[目的]研究贵州省平塘县500 m口径球面射电望远镜(FAST)工程台址光明顶滑坡的危险性及其区划,为该区及相似地区的滑坡灾害监测与防治提供参考依据。[方法]选取地形地貌、岩性岩组、地质构造、人类工程活动及灾害历史等作为一级评价指标,在此基础上发展二级评价指标,运用GIS技术构建基于模糊层次法(FAHP)的滑坡危险性评价指标体系,并完全参与对指标隶属度矩阵的运算,开展针对喀斯特单体滑坡危险性评价研究。[结果]光明顶坡体总体居于中危险和低危险水平,局部高危险,高危险区域主要位于1 H馈源塔和断层破碎带区域的3个支撑柱附近,高危险区约占研究区面积的4%。[结论]光明顶坡体存在安全隐患,已经对精密的射电望远镜设施的安全运行构成威胁。本文评价结果与实际情况基本相符,评价方法在单体滑坡危险性评价研究方面具有创新性,且可操作。     

辽西北沙地苹果-花生间作系统土壤养分空间分布特征及间作效应

[目的] 研究辽西北沙地农林复合系统土壤养分的空间分布及其效应，从土壤养分角度探讨果农间作系统中果树和农作物对土壤养分的相互作用关系，为该区农林复合系统的可持续经营提供科学合理的依据。[方法] 以苹果与花生间作、花生单作、苹果单作为研究对象，对0-60 cm土层深度，0-300 cm水平距离范围内土壤养分含量进行测定和分析。[结果] 沙地间作系统中土壤有机质、速效钾极缺乏，全氮、碱解氮很缺乏，全磷缺乏，有效磷含量中等；间作系统在水平方向上，苹果树和花生植株对总养分有机质、氮、磷的竞争激烈位点位于果树带区，对有效养分氮、磷、钾的竞争激烈位点位于近果树作物区；在垂直方向上，各养分总体表现出了表聚性，间作系统对有机质、有效磷的竞争主要位于深土层，对全磷、速效钾的竞争主要位于表土层，对全氮、碱解氮表现为合作效应，表土层效应更高；与苹果单作、花生单作相比较，间作系统速效钾和有效磷含量呈现负效应。[结论] 沙地苹果-花生间作系统土壤养分贫瘠，应在果树带区施用有机肥、磷肥，作物区施入钾肥，以减轻养分竞争，提高养分效应。     

冀西北地区不同生态系统枯落物的水文效应

[目的]揭示不同植被生态系统枯落物水文效应的差异,为张家口地区生态空间格局优化提供支撑。[方法]基于样地调查和统计分析方法,分别对冀西北地区张家口市森林、农田和草地3种生态系统的枯落物在生长季末期持水能力及其与生物量之间的关系进行分析。[结果]①森林生态系统枯落物的最大持水量(30.7t/hm~2)、有效拦蓄量(22.97t/hm~2)、有效拦蓄率(187.49%)和吸水速率(5.84g/h)4个指标均优于其他两类生态系统。②草地枯落物的各项水文指标均值皆高于农田,最大持水率甚至高于森林生态系统。③枯落物生物量与其最大持水量和有效拦蓄量呈显著正相关关系,而枯落物占生态系统总生物量的比例随总生物量增加呈幂数下降。[结论]如果造林营林方式正确,森林生态系统能够提升区域水源涵养水平,同时草地的水源涵养能力不容忽视,市域内大范围的耕地向草地扩展,将直接影响区域的水源涵养能力。以生物量表征的生态系统固碳能力与枯落物蓄水所反映的水源涵养能力之间的响应关系在生态系统不同尺度下具有不同的表现,在对区域生态空间格局进行优化时,需明确特定环境下不同生态系统类型枯落物的水源涵养能力及其变化对其他生态服务功能的影响,从而实现各生态系统平衡发展。